The International Institute
of Molecular Mechanisms and Machines
Polish Academy of Sciences
PHD STUDENT
Project: “Cellular architecture of Archaea in the context of eukaryogenesis”
Laboratory of Structural Cell Biology,
Head: Dr. Piotr Szwedziak
POSITION IN PROJECT
PhD Student
SCIENTIFIC DISCIPLINE
Structural biology, molecular biology, biochemistry,
PROJECT NAME
"ReMedy"
PROJECT LEADER
Piotr Szwedziak /Laboratory of Structural Cell Biology,
SUBPROJECT NAME
“Cellular architecture of Archaea in the context of eukaryogenesis”
PROJECT DESCRIPTION
The main objective of this project is to structurally and functionally characterize primordial membrane remodelling complexes from a newly discovered archaeal branch and compare them to their ekuryotic counterparts. This project, which makes heaby use of biochemistry, is a great opportunity to learn cryoEM. For more information please contact Dr. Piotr Szwedziak p.szwedziak@imol.institute
Click to visit project's webpage for more information on project
NUMBER OF JOB OFFERS
1
REMUNERATION (PLN/MONTH):
3 500,00 – 4 000,00 PLN net; depends on experience
JOB TYPE
Full-time position, stipend
LOCATION
Warsaw, Poland
POSITION STARTS ON
1st June 2021 or later upon mutual agreement
APPLICATION DEADLINE
19th May 2021
YOUR FUTURE KEY RESPONSIBILITIES:
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Performing experiments using biochemical, structural and molecular biology techniques (independently and in collaboration with other team members),
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Participation in experimental design and data analysis,
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Participation in the data preparation and writing of manuscripts,
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Active participation in lab meetings, scientific seminars and conferences.
IF YOU ARE:
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MSc degree (or equivalent) in molecular biology, biophysics, biochemistry or related fields of life sciences (or perspective of obtaining one),
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Solid knowledge and familiarity with laboratory techniques in at least one of the following disciplines: molecular biology, biochemistry, cell biology, genetics, transcriptomics,
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Very good command of English,
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Motivation and passion for experimental work.
... PLEASE APPLY.
And let us present the details to you.
REQUIRED DOCUMENTS
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Motivation letter,
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CV with the list of publications,
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Contact details of the scientific supervisor and other referee/s if available (preferably including direct supervisor of applicant’s MSc thesis),
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Please apply using the form below,
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Please send all the documents as a single .pdf file, in English,
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We will contact selected candidates only.
FNP PROGRAMME
Project is carried out within the International Research Agendas Programme of the Foundation for Polish Science
PhD position in electron cryomicroscopy available at the Laboratory of Structural Cell Biology
PhD position will be available in my lab, located at the Centre of New Technologies at the University of Warsaw, Poland. The successful candidate will investigate the organisation and architecture of archaeal cells at the molecular level. We utilise a multiscale approach, combining imaging across scales (X-ray, single particle cryoEM and fluorescence light microscopy bridged by electron cryotomography) with biochemistry.
The Centre of New Technologies is equipped with state-of-the-art instrumentation including: a dedicated Akta Pure system for large scale protein purification, UPLC- coupled Orbitrap for native mass spectrometry, computing cluster consisting of 646 CPUs and 366 GPUs, a Vitrobot plunge freezer, a 200 kV Glacios cryo-TEM fitted with a phase plate and a Falcon 3EC direct electron detector. Additionaly, we will have access to a Titan Krios G3i cryo-TEM equipped with a phase plate, BioQuantum energy filter and both K3 and Falcon3EC direct electron detectors located at the National Centre for Electron Cryomicroscopy in Kraków, Poland.
There are two FIB-SEM instruments (FEI Helios, Zeiss CrossBeam) at the campus that will be upgraded with a Leica cryostage to enable sample thinning prior to cryotomography imaging by micromachining of biological specimens in cryo conditions.
Piotr Szwedziak, PhD
SEND APPLICATION
PhD Student
Project: “Cellular architecture of Archaea in the context of eukaryogenesis”
Process concluded