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Mitochondria play a key role in metabolism and regulatory processes within a cell. Thus, the formation of mitochondria is essential for cellular function of every being in the eukaryotic kingdom, from unicellular organisms to mammals. Mitochondria comprise 1000-1500 cellular proteins, which are synthesized outside of the mitochondria in the cytosol.

The biogenesis of mitochondria relies on the efficient import, sorting, and maturation of proteins, all governed by conserved protein translocases and other complex biological machinery.


Our research explores novel and exciting links between protein transport mechanisms and mitochondrial protein homeostasis. We postulate the presence of unique mechanisms involved in protein biogenesis that involve crosstalk between the cytosol and mitochondrial compartments.


Our goal is to better understand the complex and dynamic processes involved in the formation of functional organelles, as well as the maintenance of cellular protein homeostasis and its failures, which result in pathology.

Research interests


Scientific Degree
Mayra Alejandra
Borrero Landazábal 
Ahmet Sadık
Ben Hur
Marins Mussulini
Puchalska Linke
Puchalska Linke
Members of the group



  • Kim, M., Serwa, R., Samluk, L., Stępkowski, T., Suppanz, I., Kodron, A.,Warscheid, B., Chacinska, A. (2023) Immunoproteasome-specific subunit PSMB9 induction is required to regulate cellular proteostasis upon mitochondrial dysfunction, Nat Communications 14(1), 4092. DOI:10.1038/s41467-023-39642-8


  • Uszczynska-Ratajczak, B., Sugunan, S., Kwiatkowska, M., Migdal, M., Carbonell-Sala, S., Sokol, A., Winata, C., Chacinska, A. (2022) Profiling subcellular localization of nuclear-encoded mitochondrial gene products in zebrafish. Life Science Alliance, 25 October 2022. doi: 10.26508/lsa.202201514

  • Chojnacka; K. J., Mohanraj; K., Callegari; S., Mussulini; B. H., Elancheliyan; P., Gosk; A., Banach; T., Góral; T., Rehling; P., Serwa; R. A., Chacińska; A. (2022). Ovarian carcinoma immunoreactive antigen-like protein 2 (OCIAD2) is a novel complex III - specific assembly factor in mitochondria.  Molecular Biology of the Cell, vol. 33, no. 4. DOI: 10.1091/mbc.E21-03-0143


  • Kodroń, A., Mussulini, B.H., Pilecka, I., Chacinska, A. (2021). The ubiquitin-proteasome system and its crosstalk with mitochondria as therapeutic targets in medicine. Pharmacological Research, 163:105248. doi: 10.1016/j.phrs.2020.105248.

  • Minji Kim, Lukasz Samluk, Tomasz Maciej Stępkowski, Ida Suppanz, Remigiusz Adam Serwa, Agata Kodroń, Bettina Warscheid, Chacinska, A. (2021) Protein homeostasis is maintained by proteasomes containing PSMB9 induced by EEF1A2 upon mitochondrial stress.  bioRxiv,

  •  Barbara Uszczynska-Ratajczak, Sreedevi Sugunan, Monika Kwiatkowska, Maciej Migdal, Silvia Carbonell-Sala , Anna Sokol, Cecilia L. Winata, Chacinska, A. (2021) Profiling subcellular localization of nuclear-encoded mitochondrial gene products in zebrafish. bioRxiv,

  •  Wasilewski, M., Chacinska, A. MIC60 relays oxidation to block mitophagy. Nat Metab. 2021 Sep;3(9):1146-1147. doi: 10.1038/s42255-021-00460-1.

  •  Turek, M., Banasiak, K., Piechota, M., Shanmugam, N., Macias, M., Śliwińska, M.A., Niklewicz, M., Kowalski, K., Nowak, N., Chacinska, A., Pokrzywa, W. Muscle-derived exophers promote reproductive fitness. EMBO Rep. 2021 Aug 4;22(8):e52071. doi: 10.15252/embr.202052071. Epub 2021 Jul 20.

  •  Nowicka, U., Kim, M.J., Chacinska, A.Suppressing toxic aggregates: let MIA do it! EMBO J. 2021 Aug 16;40(16):e109001. doi: 10.15252/embj.2021109001. Epub 2021 Jul 20.

  •  Nowicka, U., Chroscicki, P., Stroobants, K., Sladowska, M., Turek, M., Uszczynska-Ratajczak, B., Kundra, R.,  Goral, T., Perni, M., Dobson, C.M., Vendruscolo, M., Chacinska, A. (2021). Cytosolic aggregation of mitochondrial proteins disrupts cellular homeostasis by stimulating other proteins aggregation. Elife. 2021 Jul 20;10:e65484. doi: 10.7554/eLife.65484.

  •  Sladowska, M., Turek, M., Kim, M-J., Drabikowski, K., Mussulini B. H., Mohanraj, K., Serwa, R. A., Topf, U., Chacinska, A. (2021). Proteasome activity contributes to pro-survival response upon mild mitochondrial stress in Caenorhabditis elegans. PloS Biology, 19(7):e3001302. DOI: 10.1371/journal.pbio.3001302.

  •  Barchiesi; A., Bazzani; V., Jabczynska; A., Borowski; L. S., Oeljeklaus; S., Warscheid; B., Chacinska; A., Szczesny R. J., Vascotto; C. (2021). DNA Repair Protein APE1 Degrades Dysfunctional Abasic mRNA in Mitochondria Affecting Oxidative Phosphorylation. Journal of Molecular Biology, 433(18):167125. DOI: 10.1016/j.jmb.2021.167125. 

  •  Chacinska, A., Rehling; P. (2021).Molecular bases of mitochondrial disorders. FEBS Letters, 595(8):973-975. DOI: 10.1002/1873-3468.14086.



  • Barchiesi, A., Bazzan, V., Tolotto, V., Elancheliyan, P., Wasilewski, M., Chacinska, A., Vascotto, C. (2020). Mitochondrial Oxidative Stress Induces Rapid Intermembrane Space/Matrix Translocation of Apurinic/Apyrimidinic Endonuclease 1 Protein through TIM23 Complex. Journal of Molecular Biology, 432(24):166713. doi: 10.1016/j.jmb.2020.11.012

  • Turek, M., Piechota, M., Shanmugam, N., Niklewicz, M., Kowalski, K., Chacinska, A., Pokrzywa, W. (2020). Nutritional status and fecundity are synchronised by muscular exopheresis. bioRxiv,

  • Chojnacka, K. J., Mohanraj, M., Callegari, S., Mussulini B. H. M., Elanchelyan, P., Gosk, A., Banach, T., Góral, T., Rehling, P., Serwa, R. A., Chacińska, A. (2020). Ovarian carcinoma immunoreactive antigen-like protein 2 (OCIAD2) is a novel metazoan specific complex III assembly factor. bioRxiv,

  • Mohanraj, K., Nowicka, U., Chacinska, A. (2020). Mitochondrial control of cellular protein homeostasis. Biochemical Journal, 477(16), 3033-3054.

  • Kuzniewska, B., Cysewski, D., Wasilewski, M., Sakowska, P., Milek, J., Kulinski, T. M., Winiarski, M., Kozielewicz, P., Knapska, E., Dadlez, M., Chacinska, A., Dziembowski, A., Dziembowska, M. (2020). Mitochondrial protein biogenesis in the synapse is supported by local translation. EMBO reports, 21(8), e48882.

  • Pacheu-Grau, D., Wasilewski, M., Oeljeklaus, S., Gibhardt, C. S., Aich, A., Chudenkova, M., Dennerlein, S., Deckers, M., Bogeski, J., Warscheid, B., Chacinska, A., Rehling, P. (2020). COA6 Facilitates Cytochrome c Oxidase Biogenesis as Thiol-reductase for Copper Metallochaperones in Mitochondria. Journal of Molecular Biology, doi: 10.1016/j.jmb.2020.01.036.


  • Monteuuis, G., Miścicka, A., Świrski, M., Zenad, L., Niemitalo, O., Wrobel, L., Alam J., Chacinska A., Kastaniotis AJ., Kufel J.(2019). Non-canonical translation initiation in yeast generates a cryptic pool of mitochondrial proteins. Nucleic acids research, 47(11), 5777-5791. doi: 10.1093/nar/gkz301.

  • Samluk, L., Urbanska, M., Kisielewska, K., Mohanraj, K., Kim, M. J., Machnicka, K.,Liszewska E., Jaworski J., Chacinska A. (2019). Cytosolic translational responses differ under conditions of severe short-term and long-term mitochondrial stress. Molecular biology of the cell, 30(15):1864-1877. doi: 10.1091/mbc.E18-10-0628.

  • Topf, U., Uszczynska-Ratajczak, B., & Chacinska, A. (2019). Mitochondrial stress-dependent regulation of cellular protein synthesis. J Cell Sci, 132(8), DOI: 10.1242/jcs226258.

  • Pereira, G. C., Allen, W. J., Watkins, D. W., Buddrus, L., Noone, D., Liu, X., Richardson, A. P., Chacińska, A. & Collinson, I. (2019). A high-resolution luminescent assay for rapid and continuous monitoring of protein translocation across biological membranes. Journal of molecular biology, 431(8), 1689-1699. 10.1016/j.jmb.2019.03.007

  • Mohanraj, K., Wasilewski, M., Benincá, C., Cysewski, D., Poznanski, J., Sakowska, P.., Bugajska Z., Deckers M., Dennerlein S., Fernandez-Vizarra E., Rehling P., Dadlez M., Zeviani M., Chacinska A. (2019). Inhibition of proteasome rescues a pathogenic variant of respiratory chain assembly factor COA7. EMBO molecular medicine, 11(5). DOI: 10.15252/emmm.201809561



  • Schendzielorz, A. B., Bragoszewski, P., Naumenko, N., Gomkale, R., Schulz, C., Guiard, B., Chacinska A.  & Rehling, P. (2018). Motor recruitment to the TIM23 channel’s lateral gate restricts polypeptide release into the inner membrane. Nature communications, 9(1), 4028. DOI: 10.1038/s41467-018-06492-8,

  • Sokol. A.M, Uszczynska-Ratajczak B.,Collins M.M., Bazala M., Topf U., Lundegaard, P.R., Sugunan S., Guenther S., Kuenne C., Graumann J., Chan S.S.L., Stainier D.Y.R., Chacinska A. (2018). Loss of the Mia40a oxidoreductase leads to hepato-pancreatic insufficiency in zebrafish. PLoS Genet 14(11): e1007743. DOI: 10.1371/journal.pgen.1007743,

  • Kowalski L., Brągoszewski P., Khmelinskii A., Glow E., Knop M., Chacinska A. (2018). Determinants of the cytosolic turnover of mitochondrial intermembrane space proteins. BMC Biology 16:66, DOI: 10.1186/s12915-018-0536-1.

  • Samluk L., Chroscicki P., Chacinska A. (2018). Mitochondrial protein import stress and signaling. Curr. Opin. Physiol. 03, 41–48. DOI: 10.1016/j.cophys.2018.02.010,

  • Topf, U., Suppanz, I., Samluk, L., Wrobel, L., Böser, A., Sakowska, P., Knapp, B., Pietrzyk, M. K., Chacinska, A., & Warscheid, B. (2018). Quantitative proteomics identifies redox switches for global translation modulation by mitochondrially produced reactive oxygen species. Nature Communications, 9(1), 324, DOI:10.1038/s41467-017-02694-8.



  • Gold, V.A.M., Chroscicki, P., Bragoszewski, P., Chacinska, A. (2017). Visualization of cytosolic ribosomes on the surface of mitochondria by electron cryo-tomography. EMBO Reports, DOI 10.15252/embr.201744261.

  • Bragoszewski, P., Turek, M., Chacinska, A. (2017). Control of mitochondrial biogenesis and function by the ubiquitin–proteasome system. Open Biol., 7 (4) 170007, DOI: 10.1098/rsob.170007.

  • Chacinska A. (2017). Cell biology: Sort and destroy. Nature, 543, 324-325.

  • Wasilewski, M., Chojnacka, K., Chacinska, A. (2017). Protein trafficking at the crossroads to mitochondria. Biochim Biophys Acta, 1864(1), 125-137.



  • Wasilewski, M., Chojnacka, K., Chacinska, A. (2016). Białka mitochondrialne – import, eksport, degradacja. Postępy Biochemii, 62(2), 94-102.

  • Callegari, S., Richter, F., Chojnacka, K., Jans, D.C., Lorenzi, I., Pacheu-Grau, D., Jakobs, S., Lenz, C., Urlaub, H., Dudek, J., Chacinska, A., Rehling, P. (2016). TIM29 is a subunit of the human carrier translocase required for protein transport. FEBS Lett, 590(23), 4147-4158.

  • Wrobel, L., Sokol, A.M., Chojnacka, M., and Chacinska, A. (2016). The presence of disulfide bonds reveals an evolutionarily conserved mechanism involved in mitochondrial protein translocase assembly. Sci Rep., 6, 27484.

  • Topf, U., Wrobel, L., and Chacinska, A. (2016). Chatty Mitochondria: Keeping Balance in Cellular Protein Homeostasis. Trends Cell Biol, 26(8), 577-86.

  • Turakhiya, U., von der Malsburg, K., Gold, V.A., Guiard, B., Chacinska, A., van der Laan, M., Ieva, R. (2016). Protein Import by the Mitochondrial Presequence Translocase in the Absence of a Membrane Potential. J Mol Biol, 428, 1041-52.



  • Wrobel, L., Topf, U., Bragoszewski, P., Wiese, S., Sztolsztener, M.E., Oeljeklaus, S., Varabyova, A., Lirski, M., Chroscicki, P., Mroczek, S., Januszewicz, E., Dziembowski, A., Koblowska, M., Warscheid, B., Chacinska, A. (2015). Mistargeted mitochondrial proteins activate a proteostatic response in the cytosol. Nature, 524, 485-488.

  • Keatinge, M., Bui, H., Menke, A., Chen, Y.C., Sokol, A.M., Bai, Q., Ellett, F., Da Costa, M., Burke, D., Gegg, M., Trollope, L., Payne, T., McTighe, A., Mortiboys, H., de Jager, S., Nuthhall, H., Kuo, M.S., Fleming, A., Schapira, A.H., Renshaw, S.A., Highley, J.R., Chacinska, A., Panula, P., Burton, E.A., O’Neill, M.J., Bandmann, O. (2015). Glucocerebrosidase 1 deficient Danio rerio mirror key pathological aspects of human Gaucher disease and provise evidence of early microglial activation preceding alpha-synuclein-independent neuronal cell death. Hum Mol Genet, 24(23), 6640-52.

  • Sakowska P, Jans DC, Mohanraj K, Riedel D, Jakobs S, Chacinska A. The oxidation status of Mic19 regulates MICOS assembly. 2015. Mol Cell Biol, 35, 4222-4237.

  • Bragoszewski, P., Wasilewski, M., Sakowska, P., Gornicka, A., Böttinger, L., Qiu, J., Wiedemann, N., Chacinska, A. (2015). Retro-translocation of mitochondrial intermembrane space proteins. Proc Natl Acad Sci U S A. 112, 7713-7718.

  • Barchiesi, A., Wasilewski, M., Chacinska, A., Tell, G., Vascotto, C. (2015). Mitochondrial translocation of APE1 relies on the MIA pathway. Nucleic Acids Res. 43, 5451-5464.

  • Chojnacka, M., Gornicka, A., Oeljeklaus, S., Warscheid, B., Chacinska, A. (2015). Cox17 Protein Is an Auxiliary Factor Involved in the Control of the Mitochondrial Contact Site and Cristae Organizing System. J Biol Chem. 290, 15304-15312.


  • Ieva, R., Schrempp, S.G., Opaliński, L., Wollweber, F., Höß, P., Heißwolf, A.K., Gebert, M., Zhang, Y., Guiard, B., Rospert, S., Becker, T., Chacinska, A., Pfanner, N., van der Laan, M. (2014). Mgr2 functions as lateral gatekeeper for preprotein sorting in the mitochondrial inner membrane. Mol Cell 56, 641-652.

  • Gornicka, A., Bragoszewski, P., Chroscicki, P., Wenz, L.S., Schulz, C., Rehling, P., Chacinska, A. (2014). A discrete pathway for the transfer of intermembrane space proteins across the outer membrane of mitochondria . Mol Biol Cell 25, 3999-4009.

  • Melin, J., Schulz, C., Wrobel, L., Bernhard, O., Chacinska, A., Jahn, O., Schmidt, B., Rehling, P. (2014). Presequence recognition by the tom40 channel contributes to precursor translocation into the mitochondrial matrix. Mol Cell Biol 34, 3473-3485.

  • Sokol, A.M., Sztolsztener, M.E., Wasilewski, M., Heinz, E., Chacinska, A. (2014). Mitochondrial protein translocases for survival and wellbeing. FEBS Lett. 588, 2484-2485.

  • Pfanner, N., van der Laan, M., Amati, P., Capaldi, R.A., Caudy, A.A., Chacinska, A., Darshi, M., Deckers, M., Hoppins, S., Icho, T., Jakobs, S., Ji, J., Kozjak-Pavlovic, V., Meisinger, C., Odgren, P.R., Park, S.K., Rehling, P., Reichert, A.S., Sheikh, M.S., Taylor, S.S., Tsuchida, N., van der Bliek, A.M., van der Klei, I.J., Weissman, J.S., Westermann, B., Zha, J., Neupert, W., Nunnari, J. (2014). Uniform nomenclature for the mitochondrial contact site and cristae organizing system. J Cell Biol 204, 1083-1086.



  • Qiu, J., Wenz, L.S., Zerbes, R.M., Oeljeklaus, S., Bohnert, M., Stroud, D.A., Wirth, C., Ellenrieder, L., Thornton, N., Kutik, S., Wiese, S,, Schulze-Specking, A., Zufall, N., Chacinska, A., Guiard, B., Hunte, C., Warscheid, B., van der Laan, M., Pfanner, N., Wiedemann, N., and Becker, T. (2013). Coupling of mitochondrial import and export translocases by receptor-mediated supercomplex formation. Cell 154, 596-608.

  • Varabyova, A., Topf, U., Kwiatkowska, P., Wrobel, L., Kaus-Drobek, A., and Chacinska, A. (2013). Mia40 and MINOS act in parallel with Ccs1 in the biogenesis of mitochondrial Sod1. FEBS J 280, 4943-4959.

  • Bragoszewski, P., Gornicka, A., Sztolsztener, M.E., and Chacinska, A. (2013). The ubiquitin-proteasome system regulates mitochondrial intermembrane space proteins. Mol Cell Biol 33, 2136-48.

  • Wrobel, L., Trojanowska, A., Sztolsztener, M.E., and Chacinska, A. (2013). Mitochondrial protein import: Mia40 facilitates Tim22 translocation into the inner membrane of mitochondria.  Mol Biol Cell 24, 543-554.

  • Varabyova, A., Stojanovski, D., and Chacinska, A. (2013). Mitochondrial protein homeostasis. IUBMB Life 65, 191-201.

  • Sztolsztener, M.E., Brewinska, A., Guiard, B., and Chacinska, A. (2013). Disulfide bond formation: sulfhydryl oxidase ALR controls mitochondrial biogenesis of human MIA40. Traffic 14, 309-320.


  • Böttinger, L., Gornicka, A., Czerwik, T., Bragoszewski, P., Loniewska-Lwowska, A., Schulze-Specking, A., Truscott, K.N., Guiard, B., Milenkovic, D., and Chacinska, A. (2012). In vivo evidence for cooperation of Mia40 and Erv1 in the oxidation of mitochondrial proteins. Mol Biol Cell 23, 3957-3969.

  • Bohnert, M., Wenz, L.S., Zerbes, R.M., Horvath, S.E., Stroud, D.A., von der Malsburg, K., Muller, J.M., Oeljeklaus, S., Perschil, I., Warscheid, B., Chacinska, A., Veenhuis, M., van der Klei, I.J., Daum, G., Wiedemann, N., Becker, T., Pfanner, N., van der Laan, M. (2012). Role of mitochondrial inner membrane organizing system in protein biogenesis of the mitochondrial outer membrane. Mol Biol Cell 23, 3948-3956.

  • Stojanovski, D., Bragoszewski, P., and Chacinska, A. (2012). The MIA pathway: A tight bond between protein transport and oxidative folding in mitochondria. Biochim Biophys Acta 1823, 1142-1150.


  • von der Malsburg, K., Müller, J.M., Bohnert, M., Oeljeklaus, S., Kwiatkowska, P., Becker, T., Loniewska-Lwowska, A., Wiese, S., Rao, S., Milenkovic, D., Hutu, D.P., Zerbes, R.M., Schulze-Specking, A., Meyer, H.E., Martinou, J.C., Rospert, S., Rehling, P., Meisinger, C., Veenhuis, M., Warscheid, B., van der Klei, I.J., Pfanner ,N.*, Chacinska, A.*, van der Laan, M. (2011). Dual Role of Mitofilin in Mitochondrial Membrane Organization and Protein Biogenesis. Developmental Cell, 21, 694-707. (*co-corresponding author)

  • Becker, T., Wenz, L.S., Krüger, V., Lehmann, W., Müller, J.M., Goroncy, L., Zufall, N., Lithgow, T., Guiard, B., Chacinska, A., Wagner, R., Meisinger, C., Pfanner, N. (2011). The mitochondrial import protein Mim1 promotes biogenesis of multispanning outer membrane proteins. J Cell Biol 194, 387-95.

  • Schulz, C., Lytovchenko, O., Melin, J., Chacinska, A., Guiard, B., Neumann, P., Ficner, R., Jahn, O., Schmidt, B., Rehling, P. (2011). Tim50’s presequence receptor domain is essential for signal driven transport across the TIM23 complex. J Cell Biol 195, 643-56.


  • Chacinska, A., van der Laan, M., Mehnert, C.S., Guiard, B., Mick, D.U., Hutu, D.P., Truscott, K.N., Wiedemann, N., Meisinger, C., Pfanner, N., Rehling, P. (2010). Distinct forms of mitochondrial TOM-TIM supercomplexes define signal-dependent states of preprotein sorting. Mol Cell Biol 30, 307-318.

  • Voegtle, F. N., Schmidt, O., Chacinska, A., Pfanner, N., Meisinger, C. (2010). Native techniques for analysis of mitochondrial protein import. Methods Mol Biol 619, 425-436.


  • Chacinska, A., Koehler, C.M., Milenkovic, D., Lithgow, T., Pfanner, N. (2009). Importing mitochondrial proteins: machineries and mechanisms. Cell 138, 628-644.

  • Milenkovic, D., Ramming, T., Müller, J.M., Wenz, L.S., Gebert, N., Schulze-Specking, A., Stojanovski, D., Rospert, S., Chacinska, A. (2009). Identification of the signal directing Tim9 and Tim10 into the intermembrane space of mitochondria. Mol Biol Cell 20, 2530-2539.

  • Baker, M.J., Webb, C.T., Stroud, D.A., Palmer, C.S., Frazier, A.E., Guiard, B., Chacinska, A., Gulbis, J.M., Ryan M. (2009). Structural and functional requirements for activity of the Tim9-Tim10 complex in mitochondrial protein import. Mol Biol Cell 20, 769-779.


  • Stojanovski, D., Milenkovic, D., Müller, J.M., Gabriel, K., Schulze-Specking, A., Baker, M.J., Ryan, M.T., Guiard, B., Pfanner, N.,* Chacinska, A.* (2008). Mitochondrial protein import: precursor oxidation in a ternary complex with disulfide carrier and sulfhydryl oxidase. J Cell Biol 183, 195-202. (*co-corresponding author)

  • Chacinska*, A., Guiard*, B., Müller, J.M., Schulze-Specking, A., Gabriel, K., Kutik S., Pfanner N. (2008). Mitochondrial biogenesis: switching the sorting pathways of the intermembrane space receptor Mia40. J Biol Chem 283, 29723-29729. (*equal contribution).

  • Müller, J.M., Milenkovic, D., Guiard, B., Pfanner, N., Chacinska, A. (2008). Precursor oxidation by Mia40 and Erv1 promotes vectorial transport of proteins into the mitochondrial intermembrane space. Mol Biol Cell 19, 226-236.

  • Hutu, D.P., Guiard, B., Chacinska, A., Becker, D., Pfanner, N., Rehling, P., van der Laan, M. (2008). Mitochondrial protein import motor: differential role of Tim44 in the recruitment of Pam17 and J-complex to the presequence translocase. Mol Biol Cell 19, 2642-2649.

  • Gebert, N., Chacinska, A., Wagner, K., Guiard, B., Koehler, C.M., Rehling, P., Pfanner, N., Wiedemann, N. (2008). Assembly of the three small Tim proteins precedes docking to the mitochondrial carrier translocase. EMBO Rep 9, 548-554.

  • Stojanovski, D., Müller, J.M., Milenkovic, D., Guiard, B., Pfanner, N., Chacinska, A. (2008). The MIA system for protein import into the mitochondrial intermembrane space. Biochim Biophys Acta 1783, 610-617.


  • Milenkovic, D., Gabriel, K., Guiard, B., Schulze-Specking, A., Pfanner, N., Chacinska, A. (2007). Biogenesis of the essential Tim9-Tim10 chaperone complex of mitochondria: site-specific recognition of cysteine residues by the intermemembrane space receptor Mia40. J Biol Chem. 282, 22472-22480.

  • Milenkovic, D., Müller, J.M.M., Stojanovski, D., Pfanner, N., Chacinska, A. (2007). Diverse mechanisms and machineries for import of mitochondrial proteins.  Biol Chem 388, 891-897.

  • Meisinger, C., Pfannschmidt, S., Rissler, M., Milenkovic, D., Becker, t., Stojanovski, D., Youngman, M.J., Jensen, R.E., Chacinska, A., Guiard, B., Pfanner, N., Wiedemann, N. (2007). The morphology proteins Mdm12/Mmm1 function in the major beta-barrel assembly pathway of mitochondria. EMBO J 26, 2229-2239.

  • Sanjuán Szklarz, L.K., Kozjak-Pavlovic, V., Vogtle, F.N., Chacinska, A., Milenkovic, D., Vogel, S., Durr, M., Westermann, B., Guiard, B., Martinou, J.C., Borner, C., Pfanner, N., and Meisinger, C. (2007). Preprotein transport machineries of yeast mitochondrial outer membrane are not required for Bax-induced release of intermembrane space proteins. J Mol Biol 368, 44-54.

  • Gabriel, K., Milenkovic, D., Chacinska, A., Muller, J., Guiard, b., Pfanner, N., and Meisinger, C. (2007). Novel mitochondrial intermembrane space proteins as substrates of the MIA import pathway. J Mol Biol 365, 612-620.


  • Wiedemann, N., Pfanner, N., and Chacinska, A. (2006). Chaperoning through the mitochondrial intermembrane space. Mol Cell 21, 145-148.

  • Meinecke, M., Wagner, R., Kovermann, P., Guiard, B., Mick, D.U., Hutu, D.P., Voos, W., Truscott, K.N., Chacinska, A., Pfanner, N., and Rehling, P. (2006). Tim50 maintains the permeability barrier of the mitochondrial inner membrane. Science 312, 1523-1526.

  • Albrecht, R., Rehling, P., Chacinska, A., Brix, J., Cadamuro, S.A., Volkmer, R., Guiard, B., Pfanner, N., Zeth, K. (2006). Tim21 binding domain connects the preprotein translocase of both mitochondrial membranes. EMBO Rep 7, 1233-1238.

  • Rospert, S., and Chacinska, A. (2006). Distinct yet linked: chaperone networks in the eukaryotic cytosol. Genome Biol 7, 208.


  • Chacinska, A.*, Lind, M.*, Frazier, A.E., Dudek, J., Meisinger, C., Geissler, A., Sickmann, A., Meyer, H.E., Truscott, K.N., Guiard, B., Pfanner, N., and Rehling, P. (2005). Mitochondrial presequence translocase: switching between TOM tethering and motor recruitment involves Tim21 and Tim17. Cell 120, 817-829 (*equal contribution).

  • Rissler, M., Wiedemann, N., Pfannschmidt, S., Gabriel, K., Guiard, B., Pfanner, N., and Chacinska, A. (2005). The essential mitochondrial protein Erv1 cooperates with Mia40 in biogenesis of intermembrane space proteins. J Mol Biol 353, 485-492.

  • van der Laan, M.*, Chacinska, A.*, Lind, M., Perschil, I., Sickmann, A., Meyer, H.E., Guiard, B., Meisinger, C., Pfanner, N., and Rehling, P. (2005). Pam17 is required for architecture and translocation activity of the mitochondrial protein import motor. Mol Cell Biol 25, 7449-7458 (*equal contribution).

  • Sanjuán Szklarz, L.K., Guiard, B., Rissler, M., Wiedemann, N., Kozjak, V., van der Laan, M., Lohaus, C., Marcus, K., Meyer, H.E., Chacinska, A., Pfanner, N., and Meisinger, C. (2005). Inactivation of the mitochondrial heat shock protein Zim17 leads to aggregation of matrix Hsp70s followed by pleiotropic effects on morphology and protein biogenesis. J Mol Biol 351, 206-218.


  • Chacinska, A., Pfannschmidt, S., Wiedemann, N., Kozjak, V., Sanjuán Szklarz, L.K., Schulze-Specking, A., Truscott, K.N., Guiard, B., Meisinger, C., and Pfanner, N. (2004). Essential role of Mia40 in import and assembly of mitochondrial intermembrane space proteins. EMBO J 23, 3735-3746.

  • Chacinska, A., and Rehling, P. (2004). Moving proteins from the cytosol into mitochondria. Biochem Soc Trans 32, 774-776.

  • Meisinger, C., Rissler, M., Chacinska, A., Sanjuán Szklarz, L.K., Milenkovic, D., Kozjak, V., Schönfisch, B., Lohaus, C., Meyer, H.E., Yaffe, M.P., Guiard, B., Wiedemann, N., and Pfanner, N. (2004). The mitochondrial morphology protein Mdm10 functions in assembly of the preprotein translocase of the outer membrane. Developmental Cell 7, 61-71.

  • Towpik, J., Chacinska, A., Ciesla, M., Ginalski, K., and Boguta, M. (2004). Mutations in yeast MRF1 gene encoding mitochondrial release factor inhibit translation on mitochondrial ribosomes. J Biol Chem 279, 14096-14103.


  • Chacinska, A., Rehling, P., Guiard, B., Frazier, A.E., Schulze-Specking, A., Pfanner, N., Voos, W., and Meisinger, C. (2003). Mitochondrial translocation contact sites: separation of dynamic and stabilizing elements in formation of a TOM-TIM-preprotein supercomplex. EMBO J 22, 5370-5381.

  • Frazier, A.E., Chacinska, A., Truscott, K.N., Guiard, B., Pfanner, N., and Rehling, P. (2003). Mitochondria use different mechanisms for transport of multispanning membrane proteins through the intermembrane space. Mol Cell Biol 23, 7818-7828.

  • Sickmann, A., Reinders, J., Wagner, Y., Joppich, C. Zahedi, R., Meyer, H.E., Schönfisch, B., Perschil, I., Chacinska, A., Guiard, B., Rehling, P., Pfanner, N., and Meisinger, C. (2003). The proteome of Saccharomyces cerevisiae mitochondria. Proc Natl Acad Sci USA 100, 13207-13212.

  • Wiedemann, N., Kozjak, V., Chacinska, A., Schönfisch, B., Rospert, S., Ryan, M.T., Pfanner, N., and Meisinger, C. (2003). Machinery for protein sorting and assembly in the mitochondrial outer membrane. Nature 424, 565-571.


  • Geissler, A.*, Chacinska, A.*, Truscott, K.N., Wiedemann, N., Brandner, K., Sickmann, A., Meyer, H.E., Meisinger, C., Pfanner, N., and Rehling, P. (2002). The mitochondrial presequence translocase: an essential role of Tim50 in directing preproteins to the import channel. Cell 111, 507-518 (*equal contribution).

  • Chacinska, A., Pfanner, N., and Meisinger, C. (2002). How mitochondria import hydrophilic and hydrophobic proteins. Trends Cell Biol 12, 299-303.

  • Pfanner, N. and Chacinska, A. (2002). The mitochondrial import machinery: preprotein-conducting channels with binding sites for presequences. Biochim Biophys Acta 1592, 15-24.


  • Chacinska, A., Szczesniak, B., Kochneva-Pervukhova, N. V., Kushnirov, V.V., Ter-Avanesyan, M.D., and Boguta, M. (2001). Ssb1 chaperone is a [PSI+] prion-curing factor. Curr Genet 39, 62-67.


  • Chacinska, A., Boguta, M., Krzewska, J. and Rospert, S. (2000). Prion-dependent switching between respiratory competence and deficiency in the yeast nam9-1 mutant. Mol Cell Biol 20, 7220-9.

  • Chacinska, A., Boguta, M. (2000). Coupling of mitochondrial translation with the formation of respiratory complexes in yeast mitochondria. Acta Biochim Pol 47, 973-91.



  • Boguta, M., Chacinska, A., Murawski, M. and Szczesniak, B. (1997). Expression of the yeast NAM9 gene coding for mitochondrial ribosomal protein. Acta Biochim Pol 44, 251-8.


  • Gora, M., Chacinska, A., Rytka, J. and Labbe-Bois, R. (1996). Isolation and functional characterization of mutant ferrochelatases in Saccharomyces cerevisiae. Biochimie 78, 144-52.




Prof. Agnieszka Chacinska is the head of ReMedy IRAP and director of IMol PAS. Appointed by the President of PAS, she was responsible for organizing the new institute from the scratch. She graduated from the II High School of Stanisław Staszic in Starachowice., received a PhD from the Institute of Biochemistry and Biophysics PAS with Prof. Magdalena Boguta and post-doctoral training with Prof. Nikolaus Pfanner. This was  followed by a junior group leader position at the University of Freiburg, Germany. Subsequently, in 2009 she established a Laboratory of Mitochondrial Biogenesis at the International Institute of Molecular and Cell Biology in Warsaw, currently operating at IMol.

She is a corresponding member of PAS, a member of Academia Europaea, EMBO and German National Academy of Sciences Leopoldina.

Group leader’s short bio



  • Elected member of the German National Academy of Sciences Leopoldina


  • International Award of the FEBS Theodor Bücher medal „for outstanding achievements in Biochemistry and Molecular Biology or related sciences”.

  • Elected Academia Europaea member





  • Individual Award from the Rector of the University of Warsaw



  • Elected Corresponding Member of the Polish Academy of Science Award from the Prime Minister of Poland for scientific achievements,

  • Elected EMBO member,

  • Laureate, together with Peter Rehling, of Nicolaus Copernicus Polish-German Research Award given jointly by the Foundation for Polish Science (FNP) and the German Research Foundation (DFG).



  • Award from the Minister of Science and Higher Education for scientific achievements that led to the title of Professor,

  • Award from the President of Polish Academy of Science for scientific achievement.



  • Laureate of the Welcome Programme from the Foundation for Polish Science,

  • EMBO Installation Grantee.


  • Eugen-Graetz Prize for research, University of Freiburg.



  • Long-term FEBS fellowship,

  • Award for the Ph. D. thesis, Institute of Biochemistry and Biophysics.



  • Grant for young scientists from the Polish State Committee for Scientific Research.



  • Short-term FEBS fellowship.

Memberships & awards


National Science Centre, OPUS 17: „Effects of proteasome inhibition on mitochondrial homeostasis”, (09.10.2020-08.10.2023), leader: Agnieszka Chacińska

National Science Centre, OPUS 18: „Molecular mechanisms modulating the function of UCH-L1 deubiquitinase in human mitochondria”, (08.12.2020 – 07.12.2024), leader: Agnieszka Chacińska

National Science Centre, OPUS 18: „Mitochondrial import pathways of viral proteins”, (25.11.2020 – 24.11.2024), leader: Michał Wasilewski

Foundation for Polish Science, ReMedy, “Regenerative Mechanisms for Health”, International Research Agenda Unit, (01.11.2017. – 31.10.2022), leader: Agnieszka Chacińska, deputy leader: Magda Konarska

National Science Centre, MAESTRO 7: Cross-talk between the transport of mitochondrial proteins and cellular protein homeostasis, (04.2016-04.2021), leader: Agnieszka Chacińska

National Science Centre, SONATINA 5: Homeostatic plasticity of the mitochondrial proteome and lipidome upon stress, (15.12.2021 - 14.12.2024), leader: Vanessa Puchalska Linke

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